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Characterization and molecular cloning in Escherichia coli of a plasmid from the mollicute Spiroplasma citri |
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| Authors: | C Mouches G Barroso J M Bové |
| Abstract: | Two plasmids, pMH1 with 7 kilobase pairs and pM41 with 8 kilobase pairs, were purified from the plant pathogen Spiroplasma citri and characterized by restriction mapping. Upon in vitro DNA recombination with plasmid pBR328 as a vector, we have cloned pMH1 in Escherichia coli. A radioactive probe obtained upon nick translation of the recombinant plasmid was used to further characterize and compare pMH1 and pM41. |
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