Entrapment of high-molecular-mass DNA molecules in liposomes for the genetic transformation of animal cells. |
| |
| Authors: | J Szelei and E Duda |
| |
| Affiliation: | Institute of Biochemistry, MTA Biological Center, Szeged, Hungary. |
| |
| Abstract: | We modified the Ca/EDTA procedure for the production of liposomes [Papahadjopoulos, Vail, Jacobson & Poste (1975) Biochim. Biophys. Acta 394, 483-491] to entrap intact DNA molecules of very high molecular mass into large unilamellar phospholipid vesicles. The use of DNA-protein complexes and phage particles instead of naked linear DNA increases the efficiency of entrapment and protects the integrity of DNA molecules. We investigated the interaction of mammalian cells with liposome-encapsulated recombinant lambda bacteriophages carrying marker genes. The liposomes bind surprisingly fast to the cellular surface and are taken up by the cells. A significant proportion of the encapsulated DNA is transported to and soon located in or around the nuclei. Experiments prove that these liposomes can be used for the genetic transformation of mammalian cells. |
| |
| Keywords: | |
|
|
