| 双生子多巴胺D3受体基因多态性对儿童体成分的影响 |
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| 引用本文： | 刘晓敏,乌云达来,李玉玲. 双生子多巴胺D3受体基因多态性对儿童体成分的影响[J]. 人类学学报, 2022, 41(2): 274-281. DOI: 10.16359/j.1000-3193/AAS.2021.0028 |
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| 作者姓名： | 刘晓敏 乌云达来 李玉玲 |
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| 作者单位： | 内蒙古师范大学生命科学与技术学院,呼和浩特010022 |
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| 基金项目： | 国家自然科学基金项目（30960167; 31260265）; |
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| 摘 要： | 均衡的体成分构成对维持机体的健康状态具有重要作用,体成分受遗传与环境因素的共同影响。多巴胺参与摄食、运动及认知等活动的调节,多巴胺D3受体（DRD3）对多巴胺神经通路起关键调节作用,进而对摄食功能发挥作用,从而可能对体成分产生影响。为了解遗传与环境因素对双生子儿童体成分的影响,并探讨DRD3基因单核苷酸多态性（SNP）与体成分的相关性,对160对4-12岁双生子肱三头肌皮褶厚度（d1）、肩胛下皮褶厚度（d2）、髂前上棘位皮褶厚度（d3）和体质量（m）进行了测量,计算d4(d1+d2)、d5(d2/d1),体脂率（Pf）、瘦体质量（ml）;从口腔拭子中提取全基因组DNA;通过Amp FISTR Sino filerPlus试剂盒分析确定卵型;采用SNaPshot技术对DRD3基因4个SNP位点进行检测;使用Mx软件估算各指标遗传度;运用广义估计方程模型分析各指标与DRD3基因SNP的相关性。校正年龄效应后,除个别指标（d3, ml）外,男女生指标遗传度（h）学龄前期总体偏低,且某些指标（d2, d4, Pf, ml）的遗传度存在一定的性别差异。d2分别与rs324029、rs226082存在相关（P<0.05）; d3分别与rs2134655、rs226082存在相关（P<0.05）;d5分别与rs2134655、rs167771存在相关（P<0.05）;Pf分别与rs226082、rs167771存在相关（P<0.05）;ml分别与rs2134655、rs226082、rs167771存在相关（P<0.05）。本研究结果表明,遗传和环境因素对儿童体成分发育均有影响,但遗传效应可能存在一定的发育阶段和性别差异;DRD3基因SNPs与儿童的体成分可能存在一定的相关性。
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| 关 键 词： | 双生子 多巴胺D3受体基因 单核苷酸多态性 遗传度 体成分 |
| 收稿时间： | 2020-09-21 |
Influence of dopamine D3 receptor gene polymorphism on body composition in twins |
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| LIU Xiaomin,DALAI Wuyun,LI Yuling. Influence of dopamine D3 receptor gene polymorphism on body composition in twins[J]. Acta Anthropologica Sinica, 2022, 41(2): 274-281. DOI: 10.16359/j.1000-3193/AAS.2021.0028 |
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| Authors: | LIU Xiaomin DALAI Wuyun LI Yuling |
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| Affiliation: | Institute of Llife Science and Technology, Inner Mongolia Normal University, Hohhot 010022 |
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| Abstract: | Balanced body composition plays an important role in maintaining the health of the body. Body composition is affected by both genetic and environmental factors. Dopamine is involved in the regulation of food intake, movement and cognition, dopamine D3 receptor (DRD3) plays a key regulatory role in dopamine pathway, and then plays a role in feeding function, thus may affect body composition. In order to understand the influence of genetic and environmental factors on body composition of twins, and to explore the correlation between single nucleotide polymorphism (SNP) of DRD3 gene and body composition, the triceps skinfold (d1), subscapular skinfold (d2) and suprailiac skinfold (d3) and body mass(m) were measured in 160 pairs of twins aged 4-12 years, d4(d1+d2), d5(d2/d1), body fat percentage(Pf), lean body mass(ml) were calculated. The whole genome DNA was extracted from oral swab. The zygosity was determined by Amp FISTER SinofilerPlus kit. Four SNPs of DRD3 gene were detected by SNapshot technique. The heritability of each index was estimated by Mx software. The association between SNP of DRD3 gene and each index was analyzed by generalized estimating equation (GEE) model. After adjusting for the age effect, except for some indexes (d3, ml), the heritability of boys and girls in preschool is generally low, the heritability of some indexes (d2, d4, Pf, ml) showed some gender differences. There were association between d2 and rs32409, rs226082 (P<0.05), between d3 and rs2134655, rs226082 (P<0.05), between d5 and rs2134655, rs167771 (P<0.05), between Pf and rs226082, rs167771 (P<0.05), between ml and rs2134655, rs226082 and rs167771(P<0.05) respectively. The results showed that both genetic and environmental factors affected the body composition development of children, but there may be some developmental stages and gender differences in genetic effects. SNPs of DRD3 gene may be related to body composition of children. |
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| Keywords: | Twins Dopamine D3 receptor gene Single nucleotide polymorphism Heritability Body composition |
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