| 九香虫抗菌肽CcAMP1的分离纯化和抗菌活性检测 |
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| 引用本文： | 李尚伟,赵柏松,杜娟. 九香虫抗菌肽CcAMP1的分离纯化和抗菌活性检测[J]. 昆虫学报, 2015, 58(6): 610-616 |
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| 作者姓名： | 李尚伟 赵柏松 杜娟 |
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| 作者单位： | （1. 贵州大学昆虫研究所, 贵州山地农业病虫害点实验室, 贵阳 550025; 2. 贵州大学, 昆虫资源开发利用特色重点实验室, 贵阳 550025） |
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| 摘 要： | 【目的】从药用昆虫九香虫 Coridius chinensis 中分离纯化抗菌肽，为进一步开发九香虫抗菌肽资源及深入挖掘九香虫的药用功能奠定基础。【方法】用大肠杆菌Escherichia coli 和金黄色葡萄球菌 Staphylococcus aureus 混合物作诱导源刺激九香虫产生抗菌肽，对血淋巴进行提取、凝胶过滤层析、固相萃取及反相色谱纯化，活性组分经质谱测定。对分离得到的这种抗菌肽进行人工合成，并进行抗菌活性检测。【结果】本研究获得一种九香虫抗菌肽CcAMP1，由17个氨基酸残基组成，分子量为1 997.37 u，带1个正电荷，表面有5个疏水氨基酸。对人工合成的CcAMP1进行抗菌活性检测表明，该抗菌肽与九香虫血淋巴一样对金黄色葡萄球菌等革兰氏阳性菌和大肠杆菌等革兰氏阴性菌都有较好的抗菌活性，且对革兰氏阴性菌的抗菌活性更强。【结论】从九香虫中分离得到具有较强抗菌活性的阳离子抗菌肽CcAMP1，有较大的开发利用价值。
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| 关 键 词： | 九香虫 抗菌肽 抗菌活性 分离 纯化 |
Isolation,purification, and detection of the antimicrobial activity of the antimicrobial peptide CcAMP1 from Coridius chinensis (Hemiptera:Dinidoridae) |
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| LI Shang-Wei,ZHAO Bai-Song,DU Juan. Isolation,purification, and detection of the antimicrobial activity of the antimicrobial peptide CcAMP1 from Coridius chinensis (Hemiptera:Dinidoridae)[J]. Acta Entomologica Sinica, 2015, 58(6): 610-616 |
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| Authors: | LI Shang-Wei ZHAO Bai-Song DU Juan |
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| Affiliation: | (1. Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China; 2. Special Key Laboratory of Insect Resource Development and Utilization, Guizhou University, Guiyang 550025, China) |
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| Abstract: | 【Aim】 This study aims to isolate and purify antimicrobial peptides (AMPs) from an officinal insect Coridius chinensis so as to lay a foundation for further developing AMP resources and medicinal functions of this species. 【Methods】C. chinensis was induced by a mixture of Escherichia coli and Staphylococcus aureus as the source of stimulation to produce AMPs. The hemolymph of C. chinensis was extracted and purified using gel filtration chromatography, solid phase extraction, and reversed-phase high performance liquid chromatography (RP-HPLC). The active ingredient was determined by mass spectrometry to obtain an antimicrobial peptide. Then this peptide was artificially synthesized and its antimicrobial activity was detected. 【Results】 We obtained an antimicrobial peptide named CcAMP1, which consists of 17 amino acid residues, with a molecular weight of 1 997.37 u, a positive charge, and five hydrophobic amino acids on its surface. Detection of antimicrobial activity showed that the synthetic CcAMP1 had strong antimicrobial activity against both Gram-positive and Gram-negative bacteria, with stronger antimicrobial effects on Gram-negative bacteria, just like the hemolymph of C. chinensis. 【Conclusion】 The cationic antimicrobial peptide CcAMP1 acquired from C. chinensis shows strong antimicrobial activity and has a great exploitation value. |
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| Keywords: | Coridius chinensis antimicrobial peptide antimicrobial activity isolation purification |
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