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Disruption of the CAR1 Gene Encoding Arginase Enhances Freeze Tolerance of the Commercial Baker's Yeast Saccharomyces cerevisiae |
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| Authors: | Jun Shima Yuko Sakata-Tsuda Yasuo Suzuki Ryouichi Nakajima Hajime Watanabe Shinichi Kawamoto Hiroyuki Takano |
| Abstract: | The effect of intracellular charged amino acids on freeze tolerance in doughs was determined by constructing homozygous diploid arginase-deficient mutants of commercial baker's yeast. An arginase mutant accumulated higher levels of arginine and/or glutamate and showed increased leavening ability during the frozen-dough baking process, suggesting that disruption of the CAR1 gene enhances freeze tolerance. |
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