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Utilization of 5-Bromouracil by Thymineless Bacteria |
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| Authors: | Roger Hewitt Joan C. Suit Daniel Billen |
| Abstract: | Several thymineless Escherichia coli strains have been examined for their ability to replicate their deoxyribonucleic acid when bromouracil is substituted for thymine. The procedure we describe was used to identify a thymineless strain with characteristics relatively favorable to its use in bromouracil labeling experiments. In addition, mutants with an “absolute” thymine requirement could be easily distinguished from one with a “leaky” thymine requirement. |
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