ClaI. a new restriction endonuclease from Caryophanon latum L. |
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| Authors: | H Mayer, R Grosschedl, H Schü tte, G Hobom |
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| Affiliation: | H Mayer, R Grosschedl, H Schütte, and G Hobom |
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| Abstract: | From Caryophanon latum L site specific restriction endonuclease (ClaI) has been purified, which recognises tha DNA hexanucleotide palindrome 5'-A-T-C-G-A-T-3'. Staggered cleavage generates DNA restriction fragments with 5'-terminal pCG extensions. A CLaI map of bacteriophage lambda has been determined, which indicates cleavage inhibition due to adenine methylation at over lapping ClaI-GATC recognition sequences. Plasmid pBR322 is cut only once, in the tetracycline promoter region, and can, therefore, be used as a vector system for cloning fragments derived from ClaI digestions, and in addition for fragments generated by TaqI, HpaII, and several other enzymes. |
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