Effects of pronase on isolated chloroplasts |
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| Authors: | Fish L Franceschi V R Stocking C R |
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| Affiliation: | Department of Botany, University of California, Davis, California 95616. |
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| Abstract: | Subjecting isolated spinach chloroplasts to mild proteolysis (10-minute incubation at 20 C in 500 micrograms per milliliter pronase) caused chloroplast clumping but did not affect their integrity as measured by their ability to carry out light stimulated, glycerate-3-P-dependent O2 evolution. Transmission electron microscopy revealed no detectable differences between the control and treated plastids. Mild proteolysis inactivated exogenously added pyruvate kinase and should be a useful technique in certain enzyme distribution studies. |
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