Simple, Sensitive Zymogram Technique for Detection of Xylanase Activity in Polyacrylamide Gels |
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| Authors: | John C. Royer and J. P. Nakas |
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| Abstract: | A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands corresponding to enzymatic activity against an opaque background. |
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