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| 粪菌移植对高原环境下SD大鼠体质量、血常规指标及肠道菌群的影响 | |
| 引用本文： | 廖扬, 陈征, 王建梅, 等. 粪菌移植对高原环境下SD大鼠体质量、血常规指标及肠道菌群的影响[J]. 中国微生态学杂志, 2023, 35(6): 637-645. doi: 10.13381/j.cnki.cjm.202306003 |
| 作者姓名： | 廖扬 陈征 王建梅 杨英魁 柴沙驼 王迅 李生庆 马燕 崔占鸿 孙璐 王书祥 刘书杰 |
| 作者单位： | 1. 青海大学畜牧兽医科学院，青海省高原放牧家畜动物营养与饲料科学重点实验室，青海省牦牛工程技术研究中心，农业农村部青藏高原放牧牦牛藏羊动物营养与饲草料重点实验室，青海 西宁 810016; 2. 中国农业大学动物医学院; 3. 青海大学医学院高原医学研究中心 |
| 基金项目： | 高海拔地区奶牛适应性生产技术试验示范（16190318）；青海省牛产业科技创新平台项目（2130122.1779.36） |
| 摘 要： | 探究粪菌移植对低海拔动物的高原适应性的影响。 将30只雄性SD大鼠随机分为3组，每组10只，即模拟高原环境组（H组）、模拟高原环境＋抗生素预处理组（HA组）及模拟高原环境＋抗生素预处理＋高原鼢鼠粪菌组（HAM组）。粪菌移植结束，持续饲养3周后，将所有组大鼠转移至模拟海拔6 000 m环境低压氧舱饲养30 d。测定粪菌移植对SD大鼠体质量、血常规指标及肠道菌群的影响。 HAM组、HA组和H组3组大鼠间平均日采食量比较差异无统计学意义（ 通过粪菌移植能够提高进入高原环境的低海拔动物的高原适应性。 |
| 关 键 词： | 粪菌移植 采食量 体质量 血常规 16S rRNA 肠道菌群 |
| 收稿时间： | 2023-02-21 |
| 修稿时间： | 2023-05-23 |
Effects of fecal bacteria transplantation on body weight,blood routine indexes and intestinal flora of SD rats in plateau environment |
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| LIAO Yang, CHEN Zheng, WANG Jian-mei, et al. Effects of fecal bacteria transplantation on body weight, blood routine indexes and intestinal flora of SD rats in plateau environment[J]. Chinese Journal of Microecology, 2023, 35(6): 637-645. doi: 10.13381/j.cnki.cjm.202306003 | |
| Authors: | LIAO Yang CHEN Zheng WANG Jian-mei YANG Ying-kui CHAI Sha-tuo WANG Xun LI Sheng-qing MA Yan CUI Zhan-hong SUN Lu WANG Shu-xiang LIU Shu-jie |
| Affiliation: | 1. College of Animal Husbandry and Veterinary Medicine, Qinghai University, Key Laboratory of Animal Nutrition and Forage Feed for Yak and Sheep Grazing on the Qinghai-Tibet Plateau, Ministry of Agriculture and Rural Affairs, Qinghai Yak Engineering Technology Research Center, Qinghai Key Laboratory of Animal Nutrition and Feed Science for Plateau Grazing Livestock, Xining, Qinghai 810016, China |
| Abstract: | Objective To observe the effect of fecal microbiota transplantation (FMT) on plateau adaptation of low-altitude animals. MethodsThirty SD rats were randomly divided into 3 groups, 10 rats in each group, namely simulated plateau environment (H group), simulated plateau environment+antibiotic pretreatment (HA group), and simulated plateau environment+antibiotic pretreatment+plateau zokor feces bacteria (HAM group). After the FMT was completed for 3 weeks, all the groups were transferred to a hypobaric oxygen chamber at a simulated altitude of 6,000 m for 30 days after continuous feeding. The effects of FMT on body weight, blood routine indexes and intestinal flora of SD rats were determined. ResultsThere was no significant difference in average daily feed intake among HAM group, HA group, and H group (F = 2.928, P＞0.05), and the average daily weight gain in the H group was significant lower than that in HA group and HAM group respectively (F = 3.951, P＜0.05). Compared with the H group and HAM group, the number of neutrophils in the HA group increased by 7.85% and 51.27%, respectively, and the number and percentage of monocytes in the HA group were significantly higher than those in the H group and HAM group respectively (F = 5.131, F = 4.325, all P＜0.05). The dominant bacterial phyla in HAM group, HA group and H group were Bacteroidetes and Firmicutes. The relative abundance of Lachnospiraceae in the intestinal microbiota of the HAM group rats was significantly higher compared to the HA and H groups (F = 8.545, P＜0.01), and the relative abundance of Lachnospiraceae_NK4A136_group was significantly higher in the HAM group than in the HA and H groups (F = 3.926, P＜0.05). LEfSe analysis showed that the species in HAM group with a greater impact on the community structure were Lachnospirales and Lachnospiraceae. ConclusionFecal microbiota transplantation can improve the plateau adaptability of low-altitude animals entering the plateau environment. |
| Keywords: | Fecal microbiota transplantation Feed intake Body weight Blood routine 16S rRNA Gut flora |
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