| 鲍曼不动杆菌对碳青霉烯类抗生素的耐药性分析 |
| |
| 引用本文： | 高静, 刘新. 鲍曼不动杆菌对碳青霉烯类抗生素的耐药性分析[J]. 中国微生态学杂志, 2018, 30(1). |
| |
| 作者姓名： | 高静 刘新 |
| |
| 作者单位： | 沈阳医学院,沈阳医学院 |
| |
| 基金项目： | 沈阳医学院重点实验室研究基金(20135060) |
| |
| 摘 要： | 目的 了解鲍曼不动杆菌的耐药情况，并检测耐碳青霉烯类鲍曼不动杆菌的耐药基因，为指导临床合理用药、控制院内感染提供依据。方法 利用K-B法检测45株鲍曼不动杆菌临床分离株的耐药情况，通过改良Hodge试验、Carba NP试验和EDTA协同试验对多重耐药鲍曼不动杆菌的碳青霉烯酶进行表型检测，并采用PCR技术检测鲍曼不动杆菌携带OXA-23和NDM-1型耐药基因的情况。结果 45株鲍曼不动杆菌临床分离株中共筛出42株多重耐药菌株；利用改良Hodge试验和Carba NP试验检出36株碳青霉烯酶阳性菌株；采用PCR扩增出OXA-23，未扩增出NDM-1。结论 鲍曼不动杆菌耐药情况严重，且耐药基因OXA-23携带率高，治疗时应根据药敏试验结果合理用药。
|
| 关 键 词： | 鲍曼不动杆菌 碳青霉烯类抗生素 耐药性 |
The resistance of Acinetobacter baumannii to carbapenem antibiotics |
| |
| The resistance of Acinetobacter baumannii to carbapenem antibiotics[J]. Chinese Journal of Microecology, 2018, 30(1). |
| |
| Abstract: | Abstract: Objective To learn the drug resistance of Acinetobacter baumannii and detect the resistant gene of carbapenem-resistant Acinetobacter baumannii, so as to provide theoretical basis for clinical rational use of drugs and control of nosocomial infections. Methods The drug resistance of 45 strains of Acinetobacter baumannii clinical isolates were detected by using K-B method. The phenotype of carbapenemase was analyzed through modified Hodge test, Carba NP test and EDTA co-trial. The OXA-23 and NDM-1 genes in Acinetobacter baumannii were detected with PCR. Results A total of 42 multidrug-resistant strains were detected. 36 strains of carbapenemase positive strains were detected by using modified Hodge test and Carba NP test. OXA-23 gene was amplified by using PCR while no NDM-1 gene was found. Conclusion The drug resistance of Acinetobacter baumannii is very high, and most of the strains carry OXA-23 gene. Antibacterial drugs should be used reasonably based on the results of drug susceptibility test. |
| |
| Keywords: | Acinetobacter baumannii Carbapenem antibiotics Drug resistance |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《中国微生态学杂志》浏览原始摘要信息 |
|
点击此处可从《中国微生态学杂志》下载全文 |
