Analysis of Dibenzothiophene Desulfurization in a Recombinant Pseudomonas putida Strain |
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Authors: | Javier Calzada, Marí a T. Zamarro, Almudena Alc n, Victoria E. Santos, Eduardo Dí az, Jos L. Garcí a, Felix Garcia-Ochoa |
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Affiliation: | Javier Calzada, María T. Zamarro, Almudena Alcón, Victoria E. Santos, Eduardo Díaz, José L. García, and Felix Garcia-Ochoa |
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Abstract: | Biodesulfurization was monitored in a recombinant Pseudomonas putida CECT5279 strain. DszB desulfinase activity reached a sharp maximum at the early exponential phase, but it rapidly decreased at later growth phases. A model two-step resting-cell process combining sequentially P. putida cells from the late and early exponential growth phases was designed to significantly increase biodesulfurization. |
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