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Analysis of Dibenzothiophene Desulfurization in a Recombinant Pseudomonas putida Strain
Authors:Javier Calzada, Marí  a T. Zamarro, Almudena Alc  n, Victoria E. Santos, Eduardo Dí  az, Jos   L. Garcí  a,   Felix Garcia-Ochoa
Affiliation:Javier Calzada, María T. Zamarro, Almudena Alcón, Victoria E. Santos, Eduardo Díaz, José L. García, and Felix Garcia-Ochoa
Abstract:Biodesulfurization was monitored in a recombinant Pseudomonas putida CECT5279 strain. DszB desulfinase activity reached a sharp maximum at the early exponential phase, but it rapidly decreased at later growth phases. A model two-step resting-cell process combining sequentially P. putida cells from the late and early exponential growth phases was designed to significantly increase biodesulfurization.
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