Rescue of Influenza A Virus from Recombinant DNA |
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| Authors: | Ervin Fodor, Louise Devenish, Othmar G. Engelhardt, Peter Palese, George G. Brownlee, Adolfo Garcí a-Sastre |
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| Affiliation: | Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom. |
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| Abstract: | We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes. |
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