Catabolism of [4-14C]testosterone by subcellular fractions of human prostate |
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| Authors: | J. Chamberlain Nada Jagarinec P. Ofner |
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| Affiliation: | Steroid Laboratory of the Medical Services, Lemuel Shattuck Hospital, Department of Public Health of the Commonwealth of Massachusetts, Biological Research Laboratories, Harvard School of Dental Medicine, and Department of Pharmacology, Harvard Medical School, Boston, Mass. 02130, U.S.A. |
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| Abstract: | 1. Incubation conditions were established in experiments with human-prostate homogenates for almost complete conversion of [4-(14)C]testosterone into at least ten transformation products. 2. Whole homogenates of tissue with benign hypertrophy were shown to contain 3alpha-, 3beta- and 17beta-hydroxy steroid dehydrogenases, Delta(4)-3-oxo steroid 5alpha- and 5beta- reductases and unidentified hydroxylases. 3. Most of the 17beta-hydroxy steroid-dehydrogenase activity was located in the mitochondria, which showed little other activity. 4. The 3alpha- and 3beta-hydroxy steroid dehydrogenases and the 5beta-reductase were located in the high-speed supernatant and required supplementation with NADPH for activity. 5. The 5alpha-reductase was located in both microsomal and high-speed supernatant fractions and also required supplementation with NADPH. |
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