Colicin cleavage by OmpT protease during both entry into and release from Escherichia coli cells. |
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| Authors: | D Cavard and C Lazdunski |
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| Affiliation: | Centre de Biochimie et de Biologie Moléculaire, Centre National de la Recherche Scientifique, Marseille, France. |
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| Abstract: | Proteolysis of colicins A, E1, E2, and E3 was observed after they were added to whole cells carrying a functional ompT gene. Recombinant plasmid pML19 containing the ompT gene enabled two mutant strains to cleave the added colicins. On the other hand, two colicin A recombinants were split after release from the wild-type bacteria that produced them but not from ompT mutant cells. |
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