Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood. |
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| Authors: | T F Schwarz, S Serke, B Hottentr ger, A von Brunn, H Baurmann, A Kirsch, W Stolz, D Huhn, F Deinhardt, M Roggendorf |
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| Affiliation: | Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilian University, Munich, Germany. |
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| Abstract: | Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication. |
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