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| 外源谷胱甘肽对石竹幼苗镉毒害的缓解效应 | |
| 引用本文： | 丁继军,潘远智,李丽,刘柿良,崔明峰,高佩刚. 外源谷胱甘肽对石竹幼苗镉毒害的缓解效应[J]. 植物生态学报, 2013, 37(10): 950-960. DOI: 10.3724/SP.J.1258.2013.00098 |
| 作者姓名： | 丁继军 潘远智 李丽 刘柿良 崔明峰 高佩刚 |
| 作者单位： | 四川农业大学风景园林学院, 成都 611130 长江三峡实业有限公司, 湖北宜昌 443002 西南林业大学林学院, 昆明 650224 成都易合建筑景观设计有限公司, 成都 610032 |
| 摘 要： | 为了探讨外源谷胱甘肽(GSH)对地被植物镉(Cd)毒害的缓解效应, 采用温室盆栽土培的方法, 研究了不同浓度(0、20、40、60、80、100 mg·L -1)的外源GSH处理对50 mg·kg -1 Cd胁迫下石竹(Dianthus chinensis)幼苗生长的影响。结果发现, 50 mg·kg -1 Cd显著抑制了石竹幼苗的生长。喷施外源GSH后, 一定浓度范围内(≤60 mg·L -1)的外源GSH可显著缓解石竹幼苗的Cd胁迫, 过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDAR)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)的活性, 抗坏血酸(AsA)和GSH含量以及生物量、株高、分蘖数都显著高于无外源GSH处理的石竹幼苗, 而丙二醛(MDA)含量、细胞膜透性、Cd含量、O2· -的产生速率以及H2O2的积累量则显著低于无外源GSH处理的石竹幼苗, 但随着外源GSH喷施浓度的增加, 缓解效应有下降的趋势。试验表明55-65 mg·L -1的外源GSH缓解效果最佳。 |
| 关 键 词： | 抗坏血酸-谷胱甘肽循环 镉胁迫 石竹幼苗 外源谷胱甘肽 缓解效应 |
| 收稿时间： | 2013-03-07 |
Mitigation effects of exogenous glutathione on the growth of Dianthus chinensis seedlings under Cd stress |
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| DING Ji-Jun,PAN Yuan-Zhi,LI Li,LIU Shi-Liang,CUI Ming-Feng,GAO Pei-Gang. Mitigation effects of exogenous glutathione on the growth of Dianthus chinensis seedlings under Cd stress[J]. Acta Phytoecologica Sinica, 2013, 37(10): 950-960. DOI: 10.3724/SP.J.1258.2013.00098 | |
| Authors: | DING Ji-Jun PAN Yuan-Zhi LI Li LIU Shi-Liang CUI Ming-Feng GAO Pei-Gang |
| Affiliation: | College of Landscape Architecture, Sichuan Agricultural University, Chengdu 611130, China The Three Gorges Industrial Co.,Ltd., Yichang, Hubei 443002, China College of Forestry, Southwest Forestry University, Kunming 650224, China Cheng Du Yihe Landscape Architecture Design Co.Ltd., Chengdu 610032, China |
| Abstract: | Aims The objective was to investigate exogenous glutathione (GSH) mitigation effects of Cadmium (Cd) toxicity on ground cover plants. Methods A greenhouse pot experiment was conducted to explore the effects of spraying different concentrations (0, 20, 40, 60, 80 and 100 mg·L -1) exogenous GSH on Dianthus chinensis seedlings exposed to 50 mg·kg -1 Cd in the soil. Important findings 50 mg·kg -1 Cd significantly inhibited the growth of D. chinensis seedlings; however, a range of concentrations of sprayed exogenous GSH significantly relieved Cd stress on D. chinensis seedlings. Sprayed D. chinensis seedlings had increased activities of the catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GR), as well as increased ascorbic acid (AsA) and GSH contents, biomass, plant height and number of tillers. There were decreases in malondialdehyde (MDA), cell membrane permeability, Cd contents, O2· - production rates and accumulated amount of H2O2. However, with increased exogenous GSH concentration, there was a downward trend in mitigation effects. Considering all factors, the mitigation effects were maximized by spraying 55-65 mg·L -1 exogenous GSH. |
| Keywords: | AsA-GSH cycle, Cd stress, Dianthus chinensis seedling')" >Dianthus chinensis seedling, exogenous GSH, mitigation effect |
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