| 瓦氏雅罗鱼生殖洄游过程中离子调节相关生理变化研究 |
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| 引用本文： | 王瑞芳,刘玉辉,王俊,齐景伟,孟和平,高玉奎,柳玉海,蔺浩宇,安晓萍. 瓦氏雅罗鱼生殖洄游过程中离子调节相关生理变化研究[J]. 水生生物学报, 2019, 43(4): 832-840. DOI: 10.7541/2019.098 |
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| 作者姓名： | 王瑞芳 刘玉辉 王俊 齐景伟 孟和平 高玉奎 柳玉海 蔺浩宇 安晓萍 |
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| 作者单位： | 内蒙古农业大学动物科学学院;内蒙古水产技术推广站;内蒙古达里湖渔场 |
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| 摘 要： | 为了解达里湖瓦氏雅罗鱼(Leuciscus waleckii)生殖洄游过程中血清离子调节相关生理变化, 对比了达里湖和贡格尔河瓦氏雅罗鱼血清离子(Na+、K+、Cl?、Ca2+和Mg2+)水平, 鳃、肠和肾组织Na+/K+-ATPase和鳃Ca2+/Mg2+-ATPase活性、血清催乳素(PRL)、生长激素(GH)和类胰岛素生长因子-1(IGF-1)水平及鳃组织结构差异; 并利用实验生态学方法, 研究达里湖中瓦氏雅罗鱼转入贡格尔河水24h后上述生理参数的响应。研究结果显示, 与达里湖未洄游的瓦氏雅罗鱼相比, 洄游到贡格尔河后其血清Na+含量显著降低(P<0.05), Cl?含量显著升高(P<0.05), 肾脏和肠组织中Na+/K+-ATPase活性显著升高(P<0.05), 而鳃组织中Na+/K+-ATPase活性无显著变化; 血清K+、Ca2+、Mg2+水平和GH、IGF-1、PRL含量无显著变化。将达里湖瓦氏雅罗鱼转入河水中24h后, 其血清Cl?含量显著升高(P<0.05)、K+含量显著降低(P<0.05), 且在鳃、肠和肾组织中Na+/K+-ATPase及鳃Ca2+/Mg2+-ATPase活性均显著升高(P<0.05), 血清PRL和IGF-1水平显著升高(P<0.05); 比较湖中和河中瓦氏雅罗鱼鳃组织形态结构, 显示湖中瓦氏雅罗鱼鳃基底膜分布着大量黏液细胞, 洄游到河水中后黏液细胞数量明显减少, 鳃基底膜上氯细胞体积增大而数量未见明显变化。本研究结果表明: 瓦氏雅罗鱼从达里湖洄游到贡格尔河后通过提高血清PRL和IGF-1水平, 进而介导鳃、肠和肾组织中Na+/K+-ATPase活性增加, 从而维持鱼体较高或稳定的血清离子水平。
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| 关 键 词： | 瓦氏雅罗鱼 生殖洄游 离子调节 Na+/K+-ATPase 氯细胞 黏液细胞 |
| 收稿时间： | 2018-06-28 |
PHYSIOLOGICAL REGULATION OF IONIC IN AMUR IDE (LEUCISCUS WALECKII) DURING THEIR SPAWNING MIGRATION |
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| Abstract: | Dali Nor lake, one of the four lakes in Inner Mongolia Autonomous Region, China, is a typical saline-alkaline lake with high concentrations of carbonate salts (alkalinity up to 60 mmol/L, pH 9.6 and salinity 6‰). Amur ide (Leuciscus waleckii) is one of the only two existing economic fish in the Dali Nor Lake with the character of spawning migration. To investigate the osmotic and ionic regulation mechanism of the L. waleckii during spawning migration form Dali Nor lake to Gongger river, we analyzed serum ionic (Na+, K+, Cl?, Ca2+ and Mg2+) and hormone (PRL, GH and IGF-1) levels, Na+/K+-ATPase activities in gills, intestinal and kidney, Ca2+/Mg2+-ATPase activities in gills and structure of gills in L. waleckii collected form Dali Nor lake and Gongger river. In addition, above parameters were studied in L. waleckii during 24-h transfer from lake to river water. Compared to lake fish, river fish had decreased Na+, significantly increased Cl? in serum as well as significantly increased Na+/K+-ATPase activities in kidney and intestinal while no significant change in gills. There were no significant differences of K+, Ca2+, Mg2+ and hormone (GH, IGF-1 and PRL) levels in serum between lake and river fishes. During 24-h lake-to-river water transfer, Cl? increased and K+ decreased significantly in serum, Na+/K+-ATPase activities in gills, intestinal and kidney and Ca2+/Mg2+-ATPase activi-ties in gills, as well as PRL and IGF-1 levels all increased significantly (P<0.05). Histology of gills showed that mucous cell counts were significantly higher in lake fish compared to that of river fish, while branchial chloride cells (CCs) counts have no obvious difference between lake and river fish, but the size of branchial CCs on filaments slightly increased in river fish. It is concluded that L. waleckii could maintain high serum ionic levels though endocrine control of Na+, K+-ATPase in osmotic regulatory tissues during spawning migration form Dali Nor lake to Gongger river. |
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