Synthesis and secretion of hydroxyproline containing macromolecules in carrots. I. Kinetic analysis |
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| Authors: | Chrispeels M J |
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| Affiliation: | Department of Biology, John Muir College, University of California, San Diego, La Jolla, California 92037. |
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| Abstract: | When disks of carrot (Daucus carota) phloem parenchyma are incubated for 6 days there is a 10-fold increase in cell wall hydroxyproline due to the synthesis and secretion of hydroxyproline-containing macromolecules. The synthesis of these molecules and their secretion are demonstrated by measuring the kinetics of incorporation and of chase of 14C-proline and hydroxyproline in different fractions of the cytoplasm and the cell wall. The hydroxyproline-containing molecules which are secreted are associated with the membranous organelles of the cytoplasm. They can be fractionated into trichloroacetic acid-soluble and trichloroacetic acid-precipitated fractions. The properties of the trichloroacetic acid-soluble fraction associated with the membranous organelles are consistent with its role as a cell wall precursor. |
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