Cloning and nucleotide sequence of the gene encoding the Ecal DNA methyltransferase. |
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| Authors: | V Brenner P Venetianer A Kiss |
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| Affiliation: | Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged. |
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| Abstract: | The gene coding for the GGTNACC specific Ecal DNA methyltransferase (M.Ecal) has been cloned in E. coli from Enterobacter cloacae and its nucleotide sequence has been determined. The ecalM gene codes for a protein of 452 amino acids (Mr: 51,111). It was determined that M.Ecal is an adenine methyltransferase. M.Ecal shows limited amino acid sequence similarity to other adenine methyltransferases. A clone that expresses Ecal methyltransferase at high level was constructed. |
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