CLD1/SRL1 modulates leaf rolling by affecting cell wall formation,epidermis integrity and water homeostasis in rice |
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| Authors: | Wen‐Qiang Li Min‐Juan Zhang Peng‐Fei Gan Lei Qiao Shuai‐Qi Yang Hai Miao Gang‐Feng Wang Mao‐Mao Zhang Wen‐Ting Liu Hai‐Feng Li Chun‐Hai Shi Kun‐Ming Chen |
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| Affiliation: | 1. State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China;2. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China;3. Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China |
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| Abstract: | Leaf rolling is considered as one of the most important agronomic traits in rice breeding. It has been previously reported that SEMI‐ROLLED LEAF 1 (SRL1) modulates leaf rolling by regulating the formation of bulliform cells in rice (Oryza sativa); however, the regulatory mechanism underlying SRL1 has yet to be further elucidated. Here, we report the functional characterization of a novel leaf‐rolling mutant, curled leaf and dwarf 1 (cld1), with multiple morphological defects. Map‐based cloning revealed that CLD1 is allelic with SRL1, and loses function in cld1 through DNA methylation. CLD1/SRL1 encodes a glycophosphatidylinositol (GPI)‐anchored membrane protein that modulates leaf rolling and other aspects of rice growth and development. The cld1 mutant exhibits significant decreases in cellulose and lignin contents in secondary cell walls of leaves, indicating that the loss of function of CLD1/SRL1 affects cell wall formation. Furthermore, the loss of CLD1/SRL1 function leads to defective leaf epidermis such as bulliform‐like epidermal cells. The defects in leaf epidermis decrease the water‐retaining capacity and lead to water deficits in cld1 leaves, which contribute to the main cause of leaf rolling. As a result of the more rapid water loss and lower water content in leaves, cld1 exhibits reduced drought tolerance. Accordingly, the loss of CLD1/SRL1 function causes abnormal expression of genes and proteins associated with cell wall formation, cuticle development and water stress. Taken together, these findings suggest that the functional roles of CLD1/SRL1 in leaf‐rolling regulation are closely related to the maintenance of cell wall formation, epidermal integrity and water homeostasis. |
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| Keywords: | rice leaf rolling glycosylphosphatidylinositol‐anchored protein cell wall epidermis water deficit |
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