|
|||||
|
|
| 团头鲂生长相关基因在不同发育时期生长轴组织的表达分析 | |
| 引用本文： | 易少奎,高泽霞,赵鸿昊,赵玉华,王卫民. 团头鲂生长相关基因在不同发育时期生长轴组织的表达分析[J]. 水生生物学报, 2015, 39(1): 101-109. DOI: 10.7541/2015.13 |
| 作者姓名： | 易少奎 高泽霞 赵鸿昊 赵玉华 王卫民 |
| 作者单位： | 1. 华中农业大学水产学院, 农业动物遗传育种与繁育教育部重点实验室, 农业部淡水生物繁育重点实验室, 武汉 430070; 2. 淡水水产健康养殖湖北省协同创新中心, 武汉 430070 |
| 基金项目： | 现代农业产业技术体系建设专项(CARS-46-05);国家自然科学基金(31201988);湖北省国际合作项目(No.2013BHE006);中央高校基本科研业务费专项资金(2013PY066,2013PY075和2014PY013);武汉市武昌鱼繁育工程技术研究中心建设项目(2013020705010318)资助 |
| 摘 要： | 为了探讨生长相关基因对团头鲂生长发育的调控, 研究采用Real-time PCR的方法定量分析了团头鲂6个生长相关基因在其不同生长发育阶段(3、6、12月龄)相关组织(脑、肝脏、肌肉)的表达情况, 并比较了这些基因在生长快和慢两个群体的表达差异. 结果显示: GHRs基因在肝脏与肌肉中的表达量高于脑, 在6月龄表达量高于3月龄与12月龄, 生长快群体中的表达量高于生长慢群体(P0.05); IGFs基因在三个组织中均有表达, 肝脏表达量最高, 生长快群体中的表达量高于生长慢群体(P0.05). MSTN a与MSTN b基因在组织中表达模式存在差异, MSTN a在肌肉中高表达, MSTN b主要在脑与肝脏中表达. HCL聚类结果表明: 除了MSTN a基因外, 其他5个基因在生长差异的两个群体中表达量均分别聚为一支. 不同时期组织表达聚类结果表明, 除了3月龄肝脏与12月龄肌肉组织, 6个生长相关基因在不同时期的同一组织中的表达模式存在相似性. Pearson相关分析显示: GHRs与IGFs呈正相关, MSTN a基因与GHR 2、IGFs基因呈负相关, 相同基因在两个群体中呈极显著相关(P0.01). |
| 关 键 词： | 团头鲂 生长基因 表达分析 聚类 |
| 收稿时间： | 2014-02-28 |
The expression analysis of growth-related genes in growth-related tissues in differential growth groups at different developmental stages of Megalobrama amblycephala |
|
| YI Shao-kui,GAO Ze-xia,ZHAO Hong-hao,ZHAO Yu-hua,WANG Wei-min. The expression analysis of growth-related genes in growth-related tissues in differential growth groups at different developmental stages of Megalobrama amblycephala[J]. Acta Hydrobiologica Sinica, 2015, 39(1): 101-109. DOI: 10.7541/2015.13 | |
| Authors: | YI Shao-kui GAO Ze-xia ZHAO Hong-hao ZHAO Yu-hua WANG Wei-min |
| Affiliation: | 1. Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, College of Fishery, Huazhong Agricultural University, Wuhan 430070, China; 2. Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan 430070, China |
| Abstract: | In this study, we investigated the expression of growth-related genes in differential growth groups at different developmental stages of M. amblycephala. We employed real-time PCR to quantify the expression of six growth-related genes in the brain, the liver and muscles. The results revealed that GHRs genes were highly expressed in the liver and muscles, and their expression level in 6-month and 12-month-old fish was higher than that in 3-month-old fish, and the expression level in the fast-growing group was higher than that in the slow-growing group (P0.05). IGFs genes were expressed in all three tissues, and the expression in the liver was the highest. The expression of IGFs decreased as the fish grew older (P0.05). MSTN a was primarily expressed in the brain and the liver. Unlike MSTN a, MSTN b was highly expressed in the muscles. Moreover, the expression levels of MSTN genes were negatively correlated with the growth rates (P0.05). The Cluster analysis showed that except for MSTN a, the expression levels of the other five genes in the differential growth groups could be clustered. The cluster results revealed that the expression patterns were similar in different growth-related tissues at different stages, except in the liver at 3 month and the muscles at 12 month. A positive correlation between the expression levels of MSTN a and GHR 2 was observed in the present study with Pearson correlation analysis. The similar correlation was also observed between MSTN a and IGFs. Furthermore, there was a significant positive correlation between the two differential growth groups in terms of the expression of the growth-related genes (P0.01). |
| Keywords: | Megalobrama amblycephala Growth related genes Expression analysis Clustering analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《水生生物学报》浏览原始摘要信息 | |
| 点击此处可从《水生生物学报》下载全文 | |