Quantitative analysis of highly parallel transfection in cell microarrays |
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| Authors: | Baghdoyan Sandrine Roupioz Yoann Pitaval Amandine Castel David Khomyakova Elena Papine Alexandre Soussaline Françoise Gidrol Xavier |
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| Affiliation: | Sandrine Baghdoyan, Yoann Roupioz, Amandine Pitaval, David Castel, Elena Khomyakova, Alexandre Papine, Françoise Soussaline, and Xavier Gidrol |
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| Abstract: | As more genomes are sequenced, we are facing the challenge of rapidly unraveling the functions of genes. To that end, cell microarrays have recently been described that transfect thousands of nucleic acids in parallel and can be used to analyze the phenotypic consequences of such perturbations. As many parameters can influence the efficacy of transfection in such a format, we describe some important features in manufacturing cell microarrays that may improve reliability and efficiency of both plasmid DNA and siRNA transfection. We have also developed image analysis software that allows automatic detection of cell clusters, quantification of transfection efficiency and levels of expression/extinction of genes. Along with cell microarrays, this bioinformatic tool should expedite functional exploration of the human genome. |
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