Isolation and Identification of a Senescence-promoting Substance from Wormwood (Artemisia absinthium L.) |
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| Authors: | Ueda J Kato J |
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| Affiliation: | College of Integrated Arts and Sciences, University of Osaka Prefecture, Mozu-Umemachi, Sakai, Osaka, 591, Japan. |
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| Abstract: | The senescence-promoting substance of wormwood (Artemisia absinthium L.) as detected by the oat (Avena sativa L. cv “Victory”) leaf assay has been identified as (−)-methyl jasmonate, methyl (1S, 2R)-3-oxo-2-(2′-cis-pentenyl)-cyclopentane-1-acetate, by gas-liquid chromatography-mass spectrometry and optical rotatory dispersion. Its senescence-promoting effect was much stronger than that of abscisic acid, and even at such a low concentration as 1 to 2.5 micrograms per milliliter, it could completely eliminate the anti-senescence action of 2 micrograms per milliliter kinetin. Comparing the biological activity of the (−)- with the (±)-forms of methyl jasmonate, it seemed that only the (−)-form was biologically active. |
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