A chicken beta-actin gene can complement a disruption of the Saccharomyces cerevisiae ACT1 gene. |
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| Authors: | R Karlsson P Aspenstr?m A S Bystr?m |
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| Affiliation: | Department of Zoological Cell Biology, University of Stockholm, Sweden. |
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| Abstract: | Recently it was demonstrated that beta-actin can be produced in Saccharomyces cerevisiae by using the expression plasmid pY beta actin (R. Karlsson, Gene 68:249-258, 1988), and several site-specific mutants are now being produced in a protein engineering study. To establish a system with which recombinant actin mutants can be tested in vivo and thus enable a correlation to be made with functional effects observed in vitro, a yeast strain lacking endogenous yeast actin and expressing exclusively beta-actin was constructed. This strain is viable but has an altered morphology and a slow-growth phenotype and is temperature sensitive to the point of lethality at 37 degrees C. |
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