| 内生真菌感染对宿主羽茅及邻生植物抗病性的影响 |
| |
| 引用本文： | 石新建,张靖歆,秦天姿,刘金铭,高玉葆,任安芝. 内生真菌感染对宿主羽茅及邻生植物抗病性的影响[J]. 植物生态学报, 2021, 45(8): 860-869. DOI: 10.17521/cjpe.2021.0132 |
| |
| 作者姓名： | 石新建 张靖歆 秦天姿 刘金铭 高玉葆 任安芝 |
| |
| 作者单位： | 南开大学生命科学学院, 天津 300071 |
| |
| 基金项目： | 国家自然科学基金(31971425) |
| |
| 摘 要： | Epichloë内生真菌能够影响宿主植物的抗病性, 但目前关于内生真菌感染对宿主邻生植物抗病性影响的研究很少。该研究首先调查了内蒙古呼伦贝尔天然草地中植物病害发生情况, 发现在常见牧草中禾本科植物病害最为严重, 其中感染内生真菌的羽茅(Achnatherum sibiricum)的总病情指数最低。进一步比较了与羽茅邻生及非邻生植物的病害差异, 结果显示羽茅显著降低了邻生羊草(Leymus chinensis)、狼针草(Stipa baicalensis)和冰草(Agropyron cristatum)的褐斑病病情指数。为探究羽茅对邻生植物病害的影响是否与其内生真菌有关, 分别从内生真菌发酵液、离体叶片和植株层面进行了室内验证试验。结果表明: 羽茅的2种内生真菌Epichloë gansuensis和Epichloë sibiricum发酵液显著抑制了新月弯孢(Curvularia lunata)、根腐离蠕孢(Bipolaris sorokiniana)、核盘菌(Sclerotinia sclerotioru)和三叶草核盘菌(Sclerotinia trifoliorum)的生长; 对于离体叶片, E. gansuensis和E. sibiricum显著减小了宿主叶片受4种病原真菌侵染后的病斑长度。在新月弯孢和根腐离蠕孢的植株接种条件下, 内生真菌显著降低了宿主羽茅及邻生植物羊草的病害程度。该研究首次发现并验证了羽茅内生真菌对邻生禾草抵御褐斑病有一定的增益作用。
|
| 关 键 词： | 内生真菌 羽茅 邻生禾草 病原真菌 褐斑病 |
| 收稿时间： | 2021-04-08 |
Effects of endophyte infection on fungal disease resistance of Achnatherum sibiricum and non-symbiotic neighbours |
| |
| SHI Xin-Jian,ZHANG Jing-Xin,QIN Tian-Zi,LIU Jin-Ming,GAO Yu-Bao,REN An-Zhi. Effects of endophyte infection on fungal disease resistance of Achnatherum sibiricum and non-symbiotic neighbours[J]. Acta Phytoecologica Sinica, 2021, 45(8): 860-869. DOI: 10.17521/cjpe.2021.0132 |
| |
| Authors: | SHI Xin-Jian ZHANG Jing-Xin QIN Tian-Zi LIU Jin-Ming GAO Yu-Bao REN An-Zhi |
| |
| Affiliation: | College of Life Sciences, Nankai University, Tianjin 300071, China |
| |
| Abstract: | Aims Epichloë infection can affect the fungal disease resistance of host grasses. However, few studies have been reported on the effects of endophyte infection on non-symbiotic neighbours. Methods We surveyed the plant diseases in natural grassland, and compared differences of total disease index between neighbor and non-neighbor plants of Achnatherum sibiricum. Then the laboratory experiments were conducted to investigate the effects of endophyte on the growth of four pathogen species as well as the brown patch of the host and its neighbor plants. Important findings Major epidemic diseases of grasses were spot blight, rust disease and powdery mildew in Hulun Buir natural grassland. Among common herbages, the total disease index of endophyte-infected A. sibiricum was the lowest. Compared with non-neighbor plants, the brown patch disease index of Leymus chinensis, Stipa baicalensis and Agropyron cristatum was significantly reduced when neighbouring with A. sibiricum. The laboratory experiments results showed that the culture filtration of both Epichloë gansuensis and Epichloë sibiricum significantly restrained the growth of Curvularia lunata, Bipolaris sorokiniana, Sclerotinia sclerotioru and Sclerotinia trifoliorum. The two species of endophytes could reduce lesion area of detached host leaves. In the intact leaves, the endophyte reduced the disease resistance of both the host and its neighbor grasses L. chinensis to C. lunata and B. sorokiniana. This study first verified that the endophytes in A. sibiricum have a positive effect on disease resistance of neighbor grasses to brown patch. |
| |
| Keywords: | endophyte Achnatherum sibiricum neighbor grasses fungal pathogens brown patch |
|
| 点击此处可从《植物生态学报》浏览原始摘要信息 |
|
点击此处可从《植物生态学报》下载免费的PDF全文 |
|
