| 染色质可及性与植物基因表达调控 |
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| 引用本文： | 李占杰,秦源. 染色质可及性与植物基因表达调控[J]. 植物学报, 2021, 56(6): 664-675. DOI: 10.11983/CBB21115 |
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| 作者姓名： | 李占杰 秦源 |
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| 作者单位： | 1福建农林大学农学院, 作物遗传育种与综合利用教育部重点实验室, 福建省海峡植物应用系统生物学重点实验室, 福州 3500022福建农林大学生命科学学院, 福州 350002 |
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| 基金项目： | 国家自然科学基金(32100325);福建省自然科学基金(2018J01711) |
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| 摘 要： | 真核生物基因组上的核小体呈现不均匀分布, 转录活跃区域的染色质结构相对松散且易被调节蛋白结合, 这些区域的可接近程度称为染色质可及性。随着测序技术的发展, DNase-seq、ATAC-seq、MNase-seq和NOMe-seq等组学技术的应用, 全基因组范围内染色质可及性检测变得简便且高效。该文主要介绍了真核生物染色质可及性的4种基本检测方法的技术原理, 总结了核小体定位、组蛋白修饰以及转录因子结合与染色质可及性的关系, 并综述了染色质可及性参与植物生长发育和环境响应研究进展, 以期为植物领域全基因组水平染色质可及性研究、顺式调控元件挖掘及发育和环境响应过程中基因表达调控网络的解析提供借鉴。
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| 关 键 词： | 染色质可及性 组蛋白修饰 DNase-seq ATAC-seq 调控元件 |
| 收稿时间： | 2021-07-12 |
Chromatin Accessibility and the Gene Expression Regulation in Plants |
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| Zhanjie Li,Yuan Qin. Chromatin Accessibility and the Gene Expression Regulation in Plants[J]. Bulletin of Botany, 2021, 56(6): 664-675. DOI: 10.11983/CBB21115 |
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| Authors: | Zhanjie Li Yuan Qin |
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| Affiliation: | 1Fujian Provincial Key Laboratory of Haixia Applied Plant System Biology, Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China2College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | The topological organization of nucleosomes across the genome is non-uniform. While densely arranged within constitutive heterochromatin, histones are depleted at regulatory loci. Chromatin accessibility is the degree to which nuclear macromolecules are able to physically contact with regulatory DNA. Following the development of next-generation sequencing technology, a variety of quantitative methods, including DNase-seq, ATAC-seq, MNase-seq and NOMe-seq, have been developed to measure genome-wide chromatin accessibility easily and efficiently. In this review, we first introduced the technical principles of the four principal methods for measuring chromatin accessibility. And then we summarized the critical biophysical determinants of chromatin accessibility, including nucleosome occupancy, histone modification and TFs combination. Finally, we described recent advances of chromatin regulation during development and stress responses in plants. Our goal is to provide a reference for researches about genome-wide chromatin accessibility mapping, identification of cis-regulatory elements, and the dissection of epigenetic and genetic regulatory networks. |
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| Keywords: | chromatin accessibility histone modification DNase-seq ATAC-seq regulatory elements |
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