Cloning of the Rhodobacter capsulatus hemA gene. |
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| Authors: | S W Biel M S Wright A J Biel |
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| Affiliation: | Department of Microbiology, Louisiana State University, Baton Rouge 70803. |
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| Abstract: | Portions of the Rhodobacter capsulatus hemA gene have been cloned from a hemA::Tn5 insertion strain into the lambda bacteriophage derivative EMBL3. A cosmid containing the wild-type R. capsulatus hemA gene was isolated by complementation of the hemA::Tn5 mutant. The cosmid contains a 1.4-kilobase EcoRI fragment that spans the hemA::Tn5 insertion site. The entire hemA gene is contained in this fragment and the adjacent 0.6-kilobase EcoRI fragment. |
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