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棉铃虫田间种群Bt毒素Cry1Ac抗性基因频率的估算
引用本文:陈海燕,杨亦桦,武淑文,杨亚军,吴益东. 棉铃虫田间种群Bt毒素Cry1Ac抗性基因频率的估算[J]. 昆虫学报, 2007, 50(1): 25-30
作者姓名:陈海燕  杨亦桦  武淑文  杨亚军  吴益东
作者单位:(南京农业大学植物保护学院昆虫学系,农业部病虫监测与治理重点开放实验室,南京210095)
基金项目:国家自然科学基金;国家高技术研究发展计划(863计划)
摘    要:采用改进的F1筛查法检测了2005年采自华北地区的棉铃虫Helicoverpa armigera (Hübner)田间种群对Bt毒素Cry1Ac的抗性基因频率。2005年从河南安阳和河北沧县转Bt基因抗虫棉上采集二代棉铃虫卵,在室内用人工饲料饲养至2龄幼虫,用1 μg/cm2 的Cry1Ac活化毒素进行初筛,将初筛存活成虫与室内筛选的GYBT抗性品系成虫进行单对杂交,并用区分剂量(2.5 μg/cm2)对F1代进行检测。经检测,2005年河南安阳棉铃虫种群和河北沧县棉铃虫种群对Cry1Ac抗性基因频率基本一致,分别为1.4×10-3和1.5×10-3。用毒素涂表法测定了2004、2005年采自河南安阳、河北高阳、河北沧县、新疆阿克苏和新疆沙湾棉铃虫田间种群对Cry1Ac活化毒素的敏感性水平,结果表明华北棉区与新疆内陆棉区棉铃虫种群对Cry1Ac的敏感性存在一定的地区性差异(<8倍)。总体上,我国华北棉区棉铃虫种群对Cry1Ac还未产生明显抗性,抗性基因频率处于正常水平。棉铃虫对转Bt基因抗虫棉的抗性风险依然存在,需要尽快启动全国性的早期抗性检测和预警工作。

关 键 词:棉铃虫  转Bt基因棉  Bt δ-内毒素  Cry1Ac  抗性等位基因  抗性检测  
文章编号:0454-6296(2007)01-0025-06
修稿时间:2006-05-17

Estimated frequency of resistance alleles to Bt toxin Cry1Ac in the field populations of Helicoverpa armigera (Hibner) from Northern China
CHEN Hai-Yan,YANG Yi-Hua,WU Shu-Wen,YANG Ya-Jun,WU Yi-Dong. Estimated frequency of resistance alleles to Bt toxin Cry1Ac in the field populations of Helicoverpa armigera (Hibner) from Northern China[J]. Acta Entomologica Sinica, 2007, 50(1): 25-30
Authors:CHEN Hai-Yan  YANG Yi-Hua  WU Shu-Wen  YANG Ya-Jun  WU Yi-Dong
Affiliation:(Key Laboratory of Monitoring and Management of Plant Diseases and Pest Insects, Ministry of Agriculture, Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China)
Abstract:An improved F1 screen technique was used to estimate the frequency of alleles conferring resistance to Bt cotton producing the Cry1Ac toxin in field populations of the cotton bollworm Helicoverpa armigera (Hübner) collected from Northern China in 2005. Eggs were collected from Bt cotton in Anyang of Henan Province and Cangxian of Hebei Province, respectively. The collected eggs were reared with artificial diet in the laboratory. The 2nd instar larvae were subjected to a primary selection with 1 μg/cm2 activated Cry1Ac, and the survivors were individually mated with moths from a laboratory-selected resistant GYBT strain. After screening F1 with a discriminating dose of 2.5 μg/cm2 activated Cry1Ac, we estimated the field resistance frequency of 1.4×10-3 for Anyang population from Henan Province, and 1.5×10-3 for Cangxian population from Hebei Province. Susceptibility of five field populations of H. armigera collected from Northern China cotton area and Xinjiang cotton area during 2004-2005 was investigated using a surface contamination bioassay. Geographic variations (<8-fold) were found between these populations. In general, the field populations of H. armigera in Northern China have not evolved prominent resistance to Cry1Ac, and their resistance frequency to Cry1Ac is at a normal level. But Bt resistance risk still exists, and the early resistance detection and alarming system should be initiated in China as early as possible.
Keywords:Cry1Ac
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