| 185例儿童急性白血病流式细胞术免疫分型研究 |
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| 引用本文： | 吴韶清,吴洁莹,廖灿,孙新,黄以宁,许遵鹏,刘光明,叶铁真,赖冬波,李焱,区小冰,颜慕霞,张力,陈劲松. 185例儿童急性白血病流式细胞术免疫分型研究[J]. 现代生物医学进展, 2011, 11(16): 3087-3091 |
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| 作者姓名： | 吴韶清 吴洁莹 廖灿 孙新 黄以宁 许遵鹏 刘光明 叶铁真 赖冬波 李焱 区小冰 颜慕霞 张力 陈劲松 |
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| 作者单位： | 1. 广州市妇女儿童医疗中心脐血库 广东广州 510623
2. 广州市妇女儿童医疗中心血液科 广东广州 510623 |
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| 基金项目： | 广州市医药卫生科技项目，广东省医学科研基金 |
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| 摘 要： | 目的:探讨儿童急性白血病流式细胞术免疫分型的意义。方法:采用流式细胞术三色荧光标记技术和CD45/SSC双参数散点图设门,检测185例儿童急性白血病的免疫表型,对抗原表达情况进行分析。结果:流式细胞术免疫分型和FAB分型的符合率为89.19%。185例儿童急性白血病中,ALL为121例,占AL的65.41%,B-ALL为113例,主要表达B系的CD19(99.12%)、CD22(98.13%)、CD79a(96.19%)、CD10(86.73%)。T-ALL占8例;主要表达CD5(100%)、CD7(100%)、cCD3(100%)、CD8(87.5%)。AML为47例,占25.41%,主要表达CD33(93.62%)、CD15(78.72%)、CD64(76.6%)、MPO(76.6%)、CD13(74.47%)。在B-ALL,AML,T-ALL中,敏感性最高的抗体分别是CD19,CD33,CD5和CD7,特异性最强的抗体分别是CD79a,MPO,cCD3。AMLL为17例,占9.19%,其中B/M为9例,T/B为5例,T/M为3例。My十-ALL为54例,占ALL的44.63%,表达的髓系抗原为CD13、CD15、CD33、CD64。Ly+-AML为18例,占AML的38.30%,表达的淋系抗原为CD19、CD4、CD7。系列非相关抗原CD34的表达率为67.57%,HLA-DR的表达率为85.41%,CD38的表达率为80.59%,TdT的表达率为62.59%。结论:流式细胞术免疫分型在白血病分型中起重要作用,是FAB分型的补充和修正,提高了儿童急性白血病诊断的准确率有必要进一步加强流式细胞术免疫分型的标准化工作。
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| 关 键 词： | 儿童 急性白血病 流式细胞术 |
Immunophenotyping of 185 Children with Acute Leukemia by Flow Cytometry |
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| WU Shao-qing,WU Jie-ying,LIAO Can,SUN Xin,HUANG Yi-ning,XU Zun-peng,LIU Guang-ming,Ye Tie-zhen,LAI Dong-bo,LI YanQ,OU Xiao-bing,YAN Mu-xiao,ZHANG Li,CHEN Jin-song. Immunophenotyping of 185 Children with Acute Leukemia by Flow Cytometry[J]. Progress in Modern Biomedicine, 2011, 11(16): 3087-3091 |
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| Authors: | WU Shao-qing WU Jie-ying LIAO Can SUN Xin HUANG Yi-ning XU Zun-peng LIU Guang-ming Ye Tie-zhen LAI Dong-bo LI YanQ OU Xiao-bing YAN Mu-xiao ZHANG Li CHEN Jin-song |
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| Affiliation: | WU Shao-qing~1,WU Jie-ying~1,LIAO Can~1,SUN Xin~2,HUANG Yi-ning~1,XU Zun-peng~1,LIU Guang-ming~2,Ye Tie-zhen~2,LAI Dong-bo~2 LI Yan~1,OU Xiao-bing~2,YAN Mu-xiao~2,ZHANG Li~2,CHEN Jin-song~1 (1 Cord Blood Bank,Guangzhou Women and Children's Medical Center,Guangzhou 510623,China,2 Department of Hematology-Oncology,China) |
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| Abstract: | Objective:To investigate the significance of immunophenotype in children's acute leukemia via flow cytometry. Methods:Immunophenotypes were detected by three-color flow cytometry(FCM) and CD45/SSC gating in 185 cases of acute leukemia (AL) and antigentic expressions were analysed.Results:The coincidence rate was 89.19%between FAB and immunophenotype.Among the 185 cases of AL,121(65.41%) were acute lymphoblastic leukemia(ALL).In 113 cases of B-lineage ALL(B-ALL),CD19(99.12%), CD22(98.13%),CD79a(96.19%),CD10(... |
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| Keywords: | Children Acute leukemia Flow cytometry |
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