Direct observation of ligand colocalization on individual receptor molecules. |
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| Authors: | G J Schü tz, W Trabesinger, T Schmidt |
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| Affiliation: | G J Schütz, W Trabesinger, and T Schmidt |
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| Abstract: | We have exploited the novel methodology of far-field fluorescence microscopy at the single molecule level to study colocalization of two different ligand molecules on an individual receptor. The use of dual-wavelength single molecule imaging allows discrimination between isolated and colocalized ligands with an accuracy of 40 nm. In the case of very close proximity of the two ligands, below 7 nm, single pair Forster energy-transfer was observed. The latter finding unequivocally demonstrates colocalization of two ligands on an individual receptor. |
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