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Holographic imaging of unlabelled sperm cells for semen analysis: a review
Authors:Giuseppe Di Caprio  Maria Antonietta Ferrara  Lisa Miccio  Francesco Merola  Pasquale Memmolo  Pietro Ferraro  Giuseppe Coppola
Affiliation:1. +1 39 617 497 4714;2. Institute for Microelectronics and Microsystems, Unit of Naples – National Research Council, Naples, Italy;3. Rowland Institute at Harvard, Harvard University, Cambridge, MA, USA;4. Institute “E. Caianiello” – National Research Council, Pozzuoli, Italy
Abstract:Male reproductive health in both humans and animals is an important research field in biological study. In order to characterize the morphology, the motility and the concentration of the sperm cells, which are the most important parameters to feature them, digital holography demonstrated to be an attractive technique. Indeed, it is a label‐free, non‐invasive and high‐resolution method that enables the characterization of live specimen. The review is intended both for summarizing the state‐of‐art on the semen analysis and recent achievement obtained by means of digital holography and for exploring new possible applications of digital holography in this field.
figure

Quantitative phase maps of living swimming spermatozoa.

Keywords:quantitative phase microscopy  single cell analysis  fertility  in vitro fertilization
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