| 致倦库蚊对登革Ⅱ型病毒的中肠感染屏障作用 |
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| 引用本文： | 谢超,赵彤言,董言德,陆宝麟. 致倦库蚊对登革Ⅱ型病毒的中肠感染屏障作用[J]. 昆虫学报, 2002, 45(2): 160-164 |
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| 作者姓名： | 谢超 赵彤言 董言德 陆宝麟 |
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| 作者单位： | 军事医学科学院微生物流行病研究所,北京,100071 |
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| 基金项目： | 总后“九五”医学杰出中青年基金课题 |
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| 摘 要： | 为探讨致倦库蚊对登革Ⅱ型病毒的中肠感染屏障作用，通过病毒分离、逆转录聚合酶链反应、透射电镜等技术进行了相关研究。结果表明：吸食感染性血液后，登革Ⅱ型病毒能侵染白纹伊蚊中肠上皮细胞并大量复制，但不能侵染致倦库蚊中肠上皮细胞。以上研究证明致倦库蚊对登革Ⅱ型病毒存在中肠感染屏障。
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| 关 键 词： | 致倦库蚊 白纹伊蚊 登革Ⅱ型病毒 中肠感染屏障 |
| 文章编号： | 0454-6296(2002)02-0160-05 |
| 修稿时间： | 2000-07-20 |
Mesenteronal infection barrier to dengue 2 virus in Culex pipiens quinquefasciatus (Diptera: Culicidae) |
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| XIE Chao,ZHAO Tong Yan ,DONG Yan De,LU Bao Lin. Mesenteronal infection barrier to dengue 2 virus in Culex pipiens quinquefasciatus (Diptera: Culicidae)[J]. Acta Entomologica Sinica, 2002, 45(2): 160-164 |
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| Authors: | XIE Chao ZHAO Tong Yan DONG Yan De LU Bao Lin |
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| Affiliation: | XIE Chao,ZHAO Tong Yan *,DONG Yan De,LU Bao Lin |
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| Abstract: | The mesenteronal infection barrier to dengue 2 virus was examined in Culex pipiens quinquefasciatus. Following large dose oral infection, the viral replication was monitored with electron microscopy, and the viral nucleic acid was detected through RT PCR at intervals in Cx. pipiens quinquefasciatus and Aedes albopictus. The results showed that the virus was replicated rapidly in Ae. albopictus after infecting mesenteronal epithelial cells. However, dengue 2 virus could not be detected in the same cells and other tissues of Cx. pipiens quinquefasciatus, which indicated that the virus was unable to infect and reproduce in it and suggested that the mesenteronal barrier to dengue 2 virus infection exist in this species of mosquito. |
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| Keywords: | Culex pipiens quinquefasciatus Aedes albopictus dengue 2 virus mesenteronal infection barrier |
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