| 海绵共生真菌产黄青霉LS16抗菌活性物质的分离及结构鉴定 |
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| 引用本文： | 李伟,邱盼盼,张云阳,丁立建,何山. 海绵共生真菌产黄青霉LS16抗菌活性物质的分离及结构鉴定[J]. 菌物学报, 2018, 37(5): 640-648. DOI: 10.13346/j.mycosystema.170227 |
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| 作者姓名： | 李伟 邱盼盼 张云阳 丁立建 何山 |
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| 作者单位： | 1宁波大学李达三海洋生物医药研究中心 浙江 宁波 3152112 浙江省海洋生物工程重点实验室 浙江 宁波 315211 |
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| 基金项目： | 国家自然科学基金（41776168,41706167）;宁波大学科研基金（XYL17016） |
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| 摘 要： | 海洋真菌能够产生大量活性独特的次级代谢产物。为了探明海绵共生真菌产黄青霉LS16发酵液中抗副溶血弧菌Vibrio parahemolyticus的活性物质,本实验对副溶血弧菌Vibrio parahemolyticus的抑菌活性进行跟踪,采用VLC（vacuum liquid chromatography）、Sephadex LH-20柱层析、薄层层析和高效液相色谱等技术,从海绵共生真菌LS16乙酸乙酯发酵液中分离纯化得到5个化合物。进一步实验证明,化合物2具有抗副溶血弧菌Vibrio parahemolyticus活性。根据该化合物的波谱数据（1H NMR、13C NMR）对其化学结构进行鉴定,确定其分子式为C15H15NO3,为生物碱类化合物。
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| 关 键 词： | 海绵共生真菌 抑菌活性 分离纯化 结构鉴定 |
| 收稿时间： | 2017-10-31 |
Isolation and structure elucidation of antibacterial metabolites from a sponge-derived fungus Penicillium chrysogenum LS16 |
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| Wei LI,Pan-Pan QIU,Yun-Yang ZHANG,Li-Jian DING,Shan HE. Isolation and structure elucidation of antibacterial metabolites from a sponge-derived fungus Penicillium chrysogenum LS16[J]. Mycosystema, 2018, 37(5): 640-648. DOI: 10.13346/j.mycosystema.170227 |
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| Authors: | Wei LI Pan-Pan QIU Yun-Yang ZHANG Li-Jian DING Shan HE |
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| Affiliation: | 1 Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University, Ningbo, Zhejiang 315211, China2 Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, Zhejiang 315211, China |
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| Abstract: | Marine fungi are prolific in production of unique secondary metabolites with promising biological activities. In an effort to find the compounds with anti-Vibrio parahemolyticus activity from the fermentation broth of a marine fungal strain Penicillium chrysogenum LS16, the ethyl acetate extract were fractionated sequentially by silica gel VLC, Sephadex LH-20 column chromatography, and reversed-phase HPLC combined with bioassay-directed isolation. Five compounds were afforded, among them compound 2 displayed potent inhibition against Vibrio parahemolyticus. The structure of compound 2 was elucidated as an alkaloid on the basis of spectroscopic analysis (1H NMR and 13C NMR), which is in agreement with the molecular formula C15H15NO3. |
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| Keywords: | sponge-derived fungus antibacterial activity separation and purification structure identification |
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