| 毛头鬼伞子实体化学成分及抗糖尿病活性初探 |
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| 引用本文： | 胡亚平,张丙乾,张金秀,赵立强,王立安. 毛头鬼伞子实体化学成分及抗糖尿病活性初探[J]. 菌物学报, 2018, 37(3): 371-378. DOI: 10.13346/j.mycosystema.170159 |
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| 作者姓名： | 胡亚平 张丙乾 张金秀 赵立强 王立安 |
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| 作者单位： | 1.邢台医学高等专科学校基础医学部 河北 邢台 0540002.河北师范大学生命科学学院 河北 石家庄 050024 |
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| 基金项目： | 河北省科技计划重点项目（16237301D）;河北省现代农业产业技术体系创新团队项目（HBCT2013060201）;河北省高等学校科学技术研究项目（ZC2016059） |
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| 摘 要： | 从毛头鬼伞子实体中萃取得到乙醇、乙酸乙酯、石油醚3种有机提取物,采用α-葡萄糖苷酶活性抑制实验对3种有机提取物的抗糖尿病活性进行评价,结果显示,乙酸乙酯提取物对α-葡萄糖苷酶有较强的抑制活性。采用柱层析技术从乙酸乙酯提取物中分离纯化出10种化合物,经核磁等方法鉴定为：（1）顺,顺-9,12-十八(碳)二烯酸;（2）顺式-9-十八烯酸;（3）(22E,24R)-麦角甾烷-5,7,22-三烯-3β醇;（4）3β-5α-6α-22E-麦角甾-7,22-双烯-3,5,6-三醇-6-亚油酸酯;（5）3β-5α-6α-22E-麦角甾-7,22-双烯-3,5,6-三醇-6-油酸酯;（6）邻苯二甲酸二异丁酯;（7）对羟基苯乙醇;（8）4-羟基苯乙基乙酸酯;（9）3-(4-羟基-3-甲氧苯基)败脂酸;（10）N-反式-3,4亚甲二氧基肉桂酰基-3-甲氧基酪胺。对分离化合物的α-葡萄糖苷酶活性抑制实验结果显示,N-反式-3,4亚甲二氧基肉桂酰基-3-甲氧基酪胺对α-葡萄糖苷酶具有较强的抑制活性,其IC50值为4.17mg/mL。
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| 关 键 词： | 毛头鬼伞,有机提取物,柱层析,核磁共振波谱,α-葡萄糖苷酶 , |
| 收稿时间： | 2017-07-30 |
Chemical constituents of fruiting bodies of Coprinus comatus and their activities against diabetes |
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| Ya-Ping HU,Bing-Qian ZHANG,Jin-Xiu ZHANG,Li-Qiang ZHAO,Li-An WANG. Chemical constituents of fruiting bodies of Coprinus comatus and their activities against diabetes[J]. Mycosystema, 2018, 37(3): 371-378. DOI: 10.13346/j.mycosystema.170159 |
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| Authors: | Ya-Ping HU Bing-Qian ZHANG Jin-Xiu ZHANG Li-Qiang ZHAO Li-An WANG |
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| Affiliation: | 1.Department of Basic Medicine, Xingtai Medical College, Xingtai, Hebei 054000, China2.College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050024, China |
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| Abstract: | The powdered fruit bodies of Coprinus comatus were firstly extracted by ethanol and the ethanol extract was then reextracted sequentially by ethyl acetate and petroleum ether. The anti-diabetic activity of each extract was evaluated by α-glucosidase inhibition assay. The results showed that ethyl acetate extract (EAE) had better anti-diabetic activity than others. Ten compounds from EAE were isolated by column chromatography and identified by spectroscopic methods including NMR and MS. These compounds were (1) cis, cis-9,12-octadecadienoic acid, (2) cis-9-octadecenoic acid, (3) (22E,24R)-ergosterol-5,7,22- triene-3β-alcohol, (4) 3β-5α-6α-22E-ergosterol-7,22-diene-3,5,6-triol-6-linoleate, (5) 3β-5α-6α-22E-ergosterol-7,22-diene-3,5,6- triol-6-oleate, (6) diisobutyl phthalate, (7) p-hydroxy benzene ethanol, (8) 4-hydroxy phenethyl acetate, (9) 3-(4-hydroxy-3-) fatty acid, and (10) N-trans-3,4-methylenedioxy cinnamic acyl-3-methoxy tyramine. N-trans-3,4-methylenedioxy cinnamic acyl-3-methoxy tyramine had better anti-diabetic activity with IC50 value of 4.17mg/mL. |
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| Keywords: | Coprinus comatus,organic extract,column chromatography,nuclear magnetic resonance spectrum (NMR),α-glucosidase , |
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