Chlorophyll a Fluorescence Yield as a Monitor of Both Active CO(2) and HCO(3) Transport by the Cyanobacterium Synechococcus UTEX 625 |
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| Authors: | Miller A G Espie G S Canvin D T |
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| Affiliation: | Department of Biology, Queen's University, Kingston, Ontario, Canada, K7L 1N7. |
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| Abstract: | Simultaneous measurements have been made of inorganic carbon accumulation (by mass spectrometry) and chlorophyll a fluorescence yield of the cyanobacterium Synechococcus UTEX 625. The accumulation of inorganic carbon by the cells was accompanied by a substantial quenching of chlorophyll a fluorescence. The quenching occurred even when CO2 fixation was inhibited by iodoacetamide and whether the accumulation of inorganic carbon resulted from either active CO2 or HCO3− transport. Measurement of chlorophyll a fluorescence yield of cyanobacteria may prove to be a rapid and convenient means of screening for mutants of inorganic carbon accumulation. |
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