Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants |
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| Authors: | Kamionka Annette Sehnal Miriam Scholz Oliver Hillen Wolfgang |
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| Affiliation: | Lehrstuhl für Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander-Universit?t Erlangen-Nürnberg, 91058 Erlangen, Germany. |
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| Abstract: | We report a regulation system in Escherichia coli for independent regulation of two distinct reporter genes by application of Tet repressors with different specificities. One Tet repressor variant comprises wild-type tet operator (tetO) recognition and exclusive induction with the novel inducer 4-dedimethylamino-anhydrotetracycline. The other Tet repressor variant shows tetO-4C recognition and induction with tetracycline. We demonstrate that both variants are independently active in vivo and allow selective regulation of two genes in the same cell without any cross talk. |
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