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Electrical Properties of the Plasmalemma and Tonoplast in Valonia ventricosa
Authors:Davis R F
Affiliation:Department of Botany, Rutgers University, Newark, New Jersey 01702.
Abstract:Studies were made on the electric potentials of the plasmalemma (Eco) and tonoplast (Evc) in small cells (1-3 mm diameter) of Valonia ventricosa. To measure Eco, microelectrodes with long tapers were inserted into the vacuole with the path of electrode entry off-center. The microelectrode then was pushed across the vacuole and into the cytoplasm on the opposite side of the cell. A reference electrode was placed in the artificial seawater bathing the cell. A similar method was used to measure Evc except that the reference electrode was placed in the vacuole.
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