Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells. |
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| Authors: | B R Stanton S W Reid L F Parada |
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| Affiliation: | Molecular Embryology Group, NCI-Frederick Cancer Research and Development Center, Maryland 21702-1201. |
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| Abstract: | We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development. |
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