Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control |
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| Authors: | Hasan Mazahir T Friedrich Rainer W Euler Thomas Larkum Matthew E Giese Günter Both Matthias Duebel Jens Waters Jack Bujard Hermann Griesbeck Oliver Tsien Roger Y Nagai Takeharu Miyawaki Atsushi Denk Winfried |
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| Affiliation: | 1Max Planck Institute for Medical ResearchHeidelbergGermany;2Universität HeidelbergZentrum für Molekulare Biologie HeidelbergGermany;3Department of Pharmacology and Howard Hughes Medical Institute, University of CaliforniaSan Diego, CaliforniaUnited States of America;4Brain Research Institute (RIKEN)SaitamaJapan |
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| Abstract: | Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes—for the measurement of population activity, in particular—have therefore been placed on calcium indicators derived from the green fluorescent protein and their expression in (selected) neuronal populations. Calcium transients can rise within milliseconds, making them suitable as reporters of fast neuronal activity. We here report the production of stable transgenic mouse lines with two different functional calcium indicators, inverse pericam and camgaroo-2, under the control of the tetracycline-inducible promoter. Using a variety of in vitro and in vivo assays, we find that stimuli known to increase intracellular calcium concentration (somatically triggered action potentials (APs) and synaptic and sensory stimulation) can cause substantial and rapid changes in FCIP fluorescence of inverse pericam and camgaroo-2. |
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