Effect of ATPase inhibitors on cell potential and k influx in corn roots |
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| Authors: | Cheeseman J M Lafayette P R Gronewald J W Hanson J B |
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| Affiliation: | Department of Botany, University of Illinois, Urbana, Illinois 61801. |
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| Abstract: | Experiments were performed to determine the effect of plasmalemma ATPase inhibitors on cell potentials (Ψ) and K+ (86Rb) influx of corn root tissue over a wide range of K+ activity. N,N′Dicyclohexylcarbodiimide (DCCD), oligomycin, and diethylstilbestrol (DES) pretreatment greatly reduced active K+ influx and depolarized Ψ at low, but not at high, K+ activity (K°). More comprehensive studies with DCCD and anoxia showed nearly complete inhibition of the active component of K+ influx over a wide range of K°, with no effect on the apparent permeability constant. DCCD had no effect on the electrogenic component of the cell potential (Ψp) above 0.2 millimolar K°. Net proton efflux was rapidly reduced 80 to 90% by DCCD. Since tissue ATP content and respiration were only slightly affected by the DCCD-pretreatment, the inhibitions of active K+ influx and Ψp at low K° can be attributed to inhibition of the plasmalemma ATPase. |
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