Novel Primers Reveal Wider Diversity among Marine Aerobic Anoxygenic Phototrophs |
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| Authors: | Natalya Yutin, Marcelino T. Suzuki, Oded B j |
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| Affiliation: | Natalya Yutin, Marcelino T. Suzuki, and Oded Béjà |
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| Abstract: | Aerobic anoxygenic phototrophic bacteria (AAnPs) were previously proposed to account for up to 11% of marine bacterioplankton and to potentially have great ecological importance in the world's oceans. Our data show that previously used primers based on the M subunit of anoxygenic photosynthetic reaction center genes (pufM) do not comprehensively identify the diversity of AAnPs in the ocean. We have designed and tested a new set of pufM-specific primers and revealed several new AAnP variants in environmental DNA samples and genomic libraries. |
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