Morphogenesis of Newcastle Disease Virus in Chorioallantoic Membrane |
| |
| Authors: | Ursula Feller Robert M. Dougherty Henry S. Di Stefano |
| |
| Affiliation: | Departments of Anatomy and Microbiology, State University of New York, Upstate Medical Center, Syracuse, New York 13210 |
| |
| Abstract: | Chick embryo chorioallantoic membrane, infected with the Blacksburg strain of Newcastle disease virus, was examined with an electron microscope to investigate the sequence of viral-induced host cell alterations. These were evident mostly in the endodermal epithelial cells lining the allantoic sac and were divided arbitrarily into three stages. Stage 1 was characterized by commencement of cell hypertrophy and hyperplasia and presence of fewer cytoplasmic inclusion bodies normally found in the cells; in stage 2, juxtanuclear nucleocapsid-glycogen aggregates appeared, and there were increased numbers of microvilli; stage 3 was characterized by increased cytoplasmic density and evidence of viral assembly and release. The morphological features of viral assembly and the virion are also described. |
| |
| Keywords: | |
|
|
