The galactose‐binding lectin isolated from Bauhinia bauhinioides Mart seeds inhibits neutrophil rolling and adhesion via primary cytokines |
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| Authors: | Deysen Kerlla Fernandes Bezerra Girão Benildo Sousa Cavada Alana de Freitas Pires Timna Varela Martins Álvaro Xavier Franco Cecília Mendes Morais Kyria Santiago do Nascimento Plinio Delatorre Helton Colares da Silva Celso Shiniti Nagano Ana Maria Sampaio Assreuy Pedro Marcos Gomes Soares |
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| Affiliation: | 1. Departamento de Morfologia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil;2. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil;3. Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, Ceará, Brazil;4. Departamento de Biologia Molecular, Universidade Federal da Paraíba, Jo?o Pessoa, Brazil;5. Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, Brazil |
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| Abstract: | In this study, the amino acid sequence and anti‐inflammatory effect of Bauhinia bauhinioides (BBL) lectin were evaluated. Tandem mass spectrometry revealed that BBL possesses 86 amino acid residues. BBL (1 mg/kg) intravenously injected in rats 30 min prior to inflammatory stimuli inhibited the cellular edema induced by carrageenan in only the second phase (21% – 3 h, 19% – 4 h) and did not alter the osmotic edema induced by dextran. BBL also inhibited carrageenan peritoneal neutrophil migration (51%), leukocyte rolling (58%) and adhesion (68%) and the neutrophil migration induced by TNF‐α (64%). These effects were reversed by the association of BBL with galactose, demonstrating that the carbohydrate‐binding domain is essential for lectin activity. In addition, BBL reduced myeloperoxidase activity (84%) and TNF‐α (68%) and IL1‐β (47%) levels. In conclusion, the present investigation demonstrated that BBL contains highly homologous isolectins, resulting in a total of 86 amino acid residues, and exhibits anti‐inflammatory activity by inhibiting neutrophil migration by reducing TNF‐α and IL1‐β levels via the lectin domain. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | Bauhinia bauhinioides galactose‐binding lectin anti‐inflammatory effect neutrophil TNF‐α |
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