High-frequency secondary mutations after suicide-driven allelic exchange mutagenesis in extraintestinal pathogenic Escherichia coli |
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| Authors: | Johnson James R Lockman Hank A Owens Krista Jelacic Srdjan Tarr Phillip I |
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| Affiliation: | Mucosal and Vaccine Research Center, Infectious Diseases (111F), Veterans Affairs Medical Center, One Veterans Drive, Minneapolis, MN 55417, USA. johns007@umn.edu |
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| Abstract: | Frequent unintended secondary mutations occurred in extraintestinal pathogenic Escherichia coli strains CP9, CFT073, and RS218 during suicide plasmid-mediated, putatively specific deletions of hlyA, papG allele III, and iha. Pulsed-field gel electrophoresis and PCR analyses demonstrated genomic alterations and/or unintended loss of defined virulence genes (papG, the F7-2 papA allele, iutA, sat, hlyD, and cnf). Caution is warranted when attributing the observed phenotypic changes to the intended mutation. |
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