Construction of a yeast mutant lacking the mitochondrial nuclease. |
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| Authors: | H P Zassenhaus T J Hofmann R Uthayashanker R D Vincent M Zona |
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| Affiliation: | Department of Microbiology, St Louis University Medical Center, MO 63104. |
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| Abstract: | The nuclear gene from Saccharomyces cerevisiae that encodes the major mitochondrial nuclease was cloned. Gene sequences were identified from a lambda gt11 library by antibodies specific to the mitochondrial nuclease. DNA from the phage recombinant was used to isolate the entire nuclease gene from a plasmid library. Yeast strains containing the nuclease gene on a multicopy plasmid vector overproduced mitochondrial nuclease 20-40 times relative to a wild-type strain. Strains containing a null allele of the nuclease gene lacked all traces of mitochondrial nuclease. Both cell types, however, were phenotypically wild-type indicating that the nuclease is not an essential enzyme for mitochondrial function. The locus encoding the mitochondrial nuclease is termed NUC1. |
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