| 绵羊X染色体59383635位点多态性与脂尾性状的相关性分析 |
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| 引用本文： | 甘尚权 张伟 沈敏 李欢 杨井泉 梁耀伟 高磊 刘守仁 王新华. 绵羊X染色体59383635位点多态性与脂尾性状的相关性分析[J]. 遗传, 2013, 35(10): 1209-1216. DOI: 10.3724/SP.J.1005.2013.01209 |
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| 作者姓名： | 甘尚权 张伟 沈敏 李欢 杨井泉 梁耀伟 高磊 刘守仁 王新华 |
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| 作者单位： | 1. 新疆农垦科学院畜牧兽医研究所, 石河子 832000; 2. 石河子大学动物科技学院, 石河子 832003; 3. 新疆农业职业技术学院动物科技学院, 昌吉 831100 |
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| 基金项目： | 国家重点基础研究发展计划(“973”计划)前期专项项目(编号：2011CB111501)和国家自然科学基金新疆联合基金重点项目(编号：U1130302)资助 |
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| 摘 要： | 脂尾(臀)性状是绵羊逆境生存的必要性状, 其脂肪在尾臀部大量沉积的遗传特性与分子机制仍不明晰。为此, 文章以筛选的X染色体59383635位点SNP为候选分子标记, 利用PCR-SSCP技术检测该位点在我国尾型极端差异的阿勒泰羊、小尾寒羊、湖羊、中国美利奴细毛羊以及引入品种萨福克羊群体中的多态性, 并采用模型分析其与尾(臀)性状的相关性。结果表明, X染色体59383635位点T等位基因高频出现在表型分值较高的阿勒泰群体中, 而C等位基因则在瘦尾型绵羊品种中高频出现; 等位基因频率T/C的比值与尾臀表型分值相关性模型表明T/C比值随着尾臀表型分值增加呈指数倍增长。以上结果提示, 绵羊X染色体59383635位点多态性在脂尾(臀)与瘦尾绵羊群体中分布存在较大差异, 该SNP可作为一个理想的分子标记应用于高、低脂绵羊品种选育, 但其生物功能仍有待进一步深入研究。
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| 关 键 词： | 脂尾性状 阿勒泰羊 多态性 基因型 |
| 收稿时间： | 2013-07-01 |
Correlation analysis between polymorphism of the 59383635th locus on X chromosome and fat-tail trait in sheep |
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| Abstract: | Fat tail or fat rump is one of essential traits for surviving in harsh environments, and the mechanism of fat deposition and its inheritable characters in sheep are still unclear. Therefore, the 59383635th locus on X chromosome in our unpublished chip data was chosen as candidate SNP, PCR-SSCP method was used to detect genotypes in five sheep breeds which have extreme differences in tail types (Altay, Small Tail Han Sheep, Hu, Chinese Merino and Suffolk), and the mathematical model was employed to analyze the correlation between the polymorphism and the trait of fat tail or fat rump. The results in this study showed that the high frequency of allele T exists in Altay flock, and the frequency of allele C appears to be particularly high in the thin tail sheep breeds. The result of mathematical model showed that the ratio of T/C increased exponentially with the increase of phenotype score. These results suggest that there is a big difference in the SNP distribution between fat tail (rump) and thin tail sheep populations, and the SNP can be used as an ideal molecular marker in high-fat or low-fat sheep breeding. However, the biological function of the SNP remains to be further studied. |
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| Keywords: | fat tail trait Altay sheep polymorphism genotype |
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