Method for Adenosine 5′-Triphosphate Measurement on Coke Waste Activated Sludge |
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| Authors: | James Russell and Joseph J. Gauthier |
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| Abstract: | Measurement of adenosine 5′-triphosphate (ATP) in coke waste activated sludge can provide a simple method for estimating the levels of viable microbes in the sludge. However, the presence of inhibitors such as phenol in the sludge interferes when the luciferin-luciferase method is used to measure ATP. These inhibiting substances can be removed from the sludge before extraction of ATP by washing the cells with dilute sodium dodecyl sulfate. |
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