| 水稻对褐飞虱抗性相关蛋白的双向电泳分析 |
| |
| 引用本文： | 陈荣智,翁清妹,黄臻,祝莉莉,何光存. 水稻对褐飞虱抗性相关蛋白的双向电泳分析[J]. 植物学报(英文版), 2002, 44(4): 427-432 |
| |
| 作者姓名： | 陈荣智 翁清妹 黄臻 祝莉莉 何光存 |
| |
| 作者单位： | 武汉大学生命科学学院,植物发育生物学教育部重点实验室,武汉,430072 |
| |
| 基金项目： | 国家转基因植物研究和产业化专项基金 |
| |
| 摘 要： | 以药用野生稻(Oryza officinalis)的转育后代B5(高抗褐飞虱(Nilaparvata lugens Stl))与感虫品种明恢63 (Oryza sativa L.)为亲本,构建了一个重组自交系群体.通过抗褐飞虱鉴定,筛选出极端抗虫株系和极端感虫株系,运用分群分析法(bulked segregant analysis,BSA)分别建成了极端抗虫集团(resistant bulk)和极端感虫集团(susceptible bulk)的蛋白质池.利用双向电泳技术,分别分析了极端抗虫集团和极端感虫集团受虫害与未受虫害的秧苗蛋白质的变化.结果发现,虫害48 h后,感虫集团的一个分子量为40 kD的蛋白质P40 (pI=6.3)的表达明显减弱甚至消失,而在抗虫集团中,P40的表达未受影响.与褐飞虱为害后抗虫株系和感虫株系不同的生理反应相联系,推测P40与水稻受褐飞虱虫害后引起的应答反应相关.
|
| 关 键 词： | 水稻 重组自交系 褐飞虱抗性 双向电泳 |
Analysis of Resistance-related Proteins in Rice Against Brown Planthopper by Two-dimensional Electrophoresis |
| |
| CHEN Rong Zhi,WENG Qing Mei,HUANG Zhen,ZHU Li Li,HE Guang Cun. Analysis of Resistance-related Proteins in Rice Against Brown Planthopper by Two-dimensional Electrophoresis[J]. Journal of integrative plant biology, 2002, 44(4): 427-432 |
| |
| Authors: | CHEN Rong Zhi WENG Qing Mei HUANG Zhen ZHU Li Li HE Guang Cun |
| |
| Abstract: | A recombinant inbred population (RI) was constructed from a cross between B5, anintrogression line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63(O.sativa L.). The brown planthopper (BPH) resistances of RI lines were evaluated. Based on bulked segregant analysis (BSA), two protein bulks were made by extracting proteins from equally mixed seedlings of extremely resistant and susceptible plants selected from the RI population, respectively. Two dimensional electrophoresis was used to detect the changes of polypeptide pattern. Results showed that a protein P40 (pI 6.3, Mw 40 kD) was significantly reduced or vanished after BPH infestation for 48 h in the susceptible bulk, while it remained uninfluenced in the resistant bulk. In connection with the physiological changes of the resistant and susceptible lines subjected to BPH sucking, we suppose that the protein P40 is related to the interaction responses of rice plants to BPH infestation. |
| |
| Keywords: | rice recombinant inbred lines brown planthopper resistance two-dimensional electrophoresis |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《植物学报(英文版)》浏览原始摘要信息 |
|
点击此处可从《植物学报(英文版)》下载全文 |
|
