Reference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination upon differentiation |
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| Authors: | Massimiliano Pagani Maxie Rockstroh Maj Schuster Grazisa Rossetti Monica Moro Mariacristina Crosti Janina M. Tomm |
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| Affiliation: | 1. Integrative Biology Program, INGM – National Institute for Molecular Genetics, Milan, Italy;2. Department of Proteomics, Helmholtz Centre for Environmental Research ‐ UFZ, Leipzig, Germany |
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| Abstract: | The differentiation of human CD4+ T cells into T helper cell subtypes and regulatory T cells is crucial to the immune response. Among subtypes, Th1 cells are dominant, representing approximately 50% of all lymphocytes. Thus far, most global proteomic studies have used only partially purified T helper cell subpopulations and/or have employed artificial protocols for inducing specific T helper cell subtypes and/or used gel‐based approaches. These studies have shed light on molecular details of certain aspects of the proteome; nevertheless a global analysis of high purity primary naïve and Th1 cells by LC‐MS/MS is required to provide a reference dataset for proteome‐based T cell subtype characterization. The utilization of highly purified Th1 cells for a global proteome assessment and the bioinformatic comparison to naïve cells reveals changes in cell metabolism and the ubiquitination pathway upon T cell differentiation. All MS data have been deposited in the ProteomeXchange with identifier PXD001066 ( http://proteomecentral.proteomexchange.org/dataset/PXD001066 ). |
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| Keywords: | Cell biology Human T‐lymphocytes Immunology LC‐MS/MS Proteome reference map Th1‐cells |
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